Chicken Glucagon, GCG ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Glucagon,glucagon-like peptide 2; glucagon-like peptide 1; GLP1; GLP2; GRPP; glicentin-related polypeptide,GcG, preproglucagon
Search name
Chicken Glucagon ELISA Kit, Chicken glucagon-like peptide 2 ELISA Kit, Chicken glucagon-like peptide 1 ELISA Kit, Chicken GLP1 ELISA Kit, Chicken GLP2 ELISA Kit, Chicken GRPP ELISA Kit, Chicken GC ELISA Kit, Chicken glicentin-related polypeptide ELISA Kit, Chicken preproglucagon ELISA KIT,Chicken GCG ELISA KIT
Intended use
Chicken Glucagon ELISA Kit allows for the in vitro quantitative determination of chicken glucagon,Glucagon concentrations in serum, Plasma,
tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Chicken Glucagon ELISA Kit has been pre-coated with an antibody specific to Glucagon. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Glucagon and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain Glucagon, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.
The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Glucagon in the samples is then determined by
comparing the O.D. of the samples to the standard curve.
Chicken Glucagon ELISA Kit range 0.31-20ng/ml
Chicken Glucagon ELISA Kit PDF manual download