Chicken Parathyroid hormone ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY!

Alternative name

Chicken PTH ELISA Kit, Chicken Parathyroid hormone ELISA Kit, Chicken Parathormone ELISA Kit, Chicken Parathyrin ELISA Kit, Chicken prepro-PTH ELISA Kit, Chicken PTH1 ELISA Kit, Chicken parathyroid hormone 1 ELISA Kit, Chicken preproparathyroid hormone ELISA Kit

Intended use

Chicken PTH ELISA Kit allows for the in vitro quantitative determination of PTH concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to PTH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PTH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PTH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 7.80 -500pg/ml

PDF Manual download

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