Deer IGFBP-3 ELISA kit
Product Name:Deer IGFBP-3 ELISA kit
Packing:96T
Catalog No.:ELA-E0054d
Gene Name:Deer IGFBP-3
Detect Range:15.625-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Deer IGFBP-3
Alternative Name:Insulin-like growth factor binding protein 3,IGFBP-3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Deer IGFBP-3 ELISA kit allows for the in vitro quantitative determination of Deer IGFBP-3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Deer IGFBP- 3 ELISA kit has been pre-coated with an Deer IGFBP-3 antibody specific to Deer IGFBP-3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Deer IGFBP-3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Deer IGFBP-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Deer IGFBP-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Technical Principle & Methodology
The quality assurance and experimental performance of this biological reagent are rigorously optimized and standardized according to its specific technical pathway:
Double Antibody Sandwich Method
(双抗体夹心法). This ensures maximum reproducibility across clinical research and scientific workflows.