Chinese Hamster Ovary Host Cell Protein, CHO HCP ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

CHO HCP, CHO-HCP,Hamster Ovary Host Cell Proteins, HCP

Search name

CHO HCP ELISA KIT, CHO-HCP ELISA KIT,Chinese Hamster Ovary Host Cell Proteins ELISA KIT, HCP ELISA KIT, Chinese Hamster Ovary Host Cell Protein ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Chinese Hamster Ovary (CHO) Host Cell Proteins concentrations in products manufactured by recombinant expression in CHO host cells.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to CHO Host Cell Proteins. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CHO Host Cell Proteins and Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CHO Host Cell Proteins, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CHO Host Cell Proteins in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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