Human 25- Dihydroxy vitamin D3, 25- HVD3 ELISA Kit

96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Calcitriol,1,25-dihydroxycholecalciferol,1,25-dihydroxyvitamin D3,

Search name
Human Calcitriol ELISA Kit, Calcitriol ELISA Kit, Human 1,25-dihydroxyvitamin D3 ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of general calcitriol,Calcitriol concentrations in serum, plasma,
tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Calcitriol, During the reaction, Calcitriol in the sample or standard competes with a fixed amount of
biotin-labeled Calcitriol for sites on a pre-coated Monoclonal antibody specific to Calcitriol. Excess conjugate and unbound sample or
standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and
incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a
sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of
Calcitriol in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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