Human 39S ribosomal protein L53, mitochondrial, MRPL53 ELISA KIT
Product Name:Human 39S ribosomal protein L53, mitochondrial, MRPL53 ELISA KIT
Packing:96T
Catalog No.:ELI-30239h
Gene Name:MRPL53
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:39S ribosomal protein L53(mitochondrial(MRPL53
Alternative Name:MRPL53,39S ribosomal protein L53(mitochondrial(MRPL53
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human 39S ribosomal protein L53, mitochondrial, MRPL53 ELISA KIT allows for the in vitro quantitative determination of 39S ribosomal protein L53(mitochondrial(MRPL53 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human 39S ribosomal protein L53, mitochondrial, MRPL53 ELISA KIT has been pre-coated with an 39S ribosomal protein L53(mitochondrial(MRPL53 antibody specific to 39S ribosomal protein L53(mitochondrial(MRPL53 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for 39S ribosomal protein L53(mitochondrial(MRPL53 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain 39S ribosomal protein L53(mitochondrial(MRPL53, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of 39S ribosomal protein L53(mitochondrial(MRPL53 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!