Human 5'- 3' exoribonuclease 1, XRN1 ELISA KIT

Product Name:Human 5'- 3' exoribonuclease 1, XRN1 ELISA KIT
Packing:96T

Catalog No.:ELI-14979h

Gene Name:XRN1

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:5'-3' exoribonuclease 1(XRN1

Alternative Name:XRN1,5'-3' exoribonuclease 1(XRN1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human 5'- 3' exoribonuclease 1, XRN1 ELISA KIT allows for the in vitro quantitative determination of 5'-3' exoribonuclease 1(XRN1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human 5'- 3' exoribonuclease 1, XRN1 ELISA KIT has been pre-coated with an 5'-3' exoribonuclease 1(XRN1 antibody specific to 5'-3' exoribonuclease 1(XRN1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for 5'-3' exoribonuclease 1(XRN1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain 5'-3' exoribonuclease 1(XRN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of 5'-3' exoribonuclease 1(XRN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!