Human 78 kDa glucose- regulated protein, HSPA5 ELISA KIT

Product Name:Human 78 kDa glucose- regulated protein, HSPA5 ELISA KIT
Packing:96T

Catalog No.:ELI-05077h

Gene Name:HSPA5

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:78 kDa glucose-regulated protein(HSPA5

Alternative Name:HSPA5,78 kDa glucose-regulated protein(HSPA5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human 78 kDa glucose- regulated protein, HSPA5 ELISA KIT allows for the in vitro quantitative determination of 78 kDa glucose-regulated protein(HSPA5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human 78 kDa glucose- regulated protein, HSPA5 ELISA KIT has been pre-coated with an 78 kDa glucose-regulated protein(HSPA5 antibody specific to 78 kDa glucose-regulated protein(HSPA5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for 78 kDa glucose-regulated protein(HSPA5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain 78 kDa glucose-regulated protein(HSPA5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of 78 kDa glucose-regulated protein(HSPA5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!