Human ACE ELISA Kit
Product Name:Human ACE ELISA Kit
Packing:96T
Catalog No.:ELA-E0004h
Gene Name:ACE
Detect Range:0.156-10 ng/mL
Sensitivity:0.067ng/mL
Target Protein Name:Human Angiotensin-converting enzyme
Alternative Name:Angiotensin-converting enzyme, ACE, Dipeptidyl carboxypeptidase I, Kininase II, ACE, DCP, DCP1, CD143
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human ACE ELISA Kit allows for the in vitro quantitative determination of Human Angiotensin-converting enzyme concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human ACE ELISA Kit has been pre-coated with an ACE antibody specific to Human Angiotensin-converting enzyme .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Angiotensin-converting enzyme and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Angiotensin-converting enzyme, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm