Human Aconitate hydratase mitochondrial, ACO2 ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

Human ACO2 ELISA KIT ,Human ACONM ELISA KIT ,Human HEL-S-284 ELISA KIT ,Human ICRD ELISA KIT ,Human OCA8 ELISA KIT ,Human OPA9 ELISA KIT ,Human aconitase 2 ELISA KIT ,Human  mitochondrial ELISA KIT ,Human citrate hydro-lyase ELISA KIT ,Human epididymis secretory sperm binding protein Li 284 ELISA KIT ,Human mitochondrial aconitase ELISA KIT ,Human aconitase 2 ELISA KIT

Intended use

Human ACO2 ELISA kit allows for the in vitro quantitative determination of Human ACO2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Human ACO2 ELISA kit has been pre-coated with an antibody specific to ACO2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ACO2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ACO2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ACO2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 0.15-10ng/ml

Human ACO2 ELISA kit Manual download

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