Human ADAMTS9 (A disintegrin and metalloproteinase with thrombospondin motifs 9)ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

Human ADAMTS9 ELISA KIT ,Human ADAM metallopeptidase with thrombospondin type 1 motif 9 ELISA KIT ,Human a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 9 ELISA KIT ,Human ADAM metallopeptidase with thrombospondin type 1 motif 9 ELISA KIT

Intended use

human ADAMTS9 ELISA kit allows for the in vitro quantitative determination of human ADAMTS9 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

Human ADAMTS9 ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ADAMTS9 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ADAMTS9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ADAMTS9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Human ADAMTS9 ELISA kit Range 0.31-20ng/ml

Human ADAMTS9 ELISA kit manual download

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