Human ADC ELISA Kit
Product Name:Human ADC ELISA Kit
Packing:96T
Catalog No.:ELA-E9547h
Gene Name:ADC
Detect Range:0.78-50 ng/mL
Sensitivity:10pg/mL
Target Protein Name:Human Arginine decarboxylase
Alternative Name:Arginine decarboxylase, ADC, ARGDC, ODC-paralogue, ODC-p, Ornithine decarboxylase-like protein, ADC, KIAA1945, ODCP
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human ADC ELISA Kit allows for the in vitro quantitative determination of Human Arginine decarboxylase concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human ADC ELISA Kit has been pre-coated with an ADC antibody specific to Human Arginine decarboxylase .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Arginine decarboxylase and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Arginine decarboxylase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Arginine decarboxylase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!