Human ADCYAP1 ELISA Kit

Product Name:Human ADCYAP1 ELISA Kit
Packing:96T

Catalog No.:ELA-E1347h

Gene Name:ADCYAP1

Detect Range:7.8-500 pg/mL

Sensitivity:2.1pg/mL

Target Protein Name:Human Pituitary adenylate cyclase-activating polypeptide

Alternative Name:Pituitary adenylate cyclase-activating polypeptide, PACAP, ADCYAP1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human ADCYAP1 ELISA Kit allows for the in vitro quantitative determination of Human Pituitary adenylate cyclase-activating polypeptide concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human ADCYAP1 ELISA Kit has been pre-coated with an ADCYAP1 antibody specific to Human Pituitary adenylate cyclase-activating polypeptide .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Pituitary adenylate cyclase-activating polypeptide and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Pituitary adenylate cyclase-activating polypeptide, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm