Human Adenylate cyclase type 9, ADCY9 ELISA KIT
Product Name:Human Adenylate cyclase type 9, ADCY9 ELISA KIT
Packing:96T
Catalog No.:ELI-49659h
Gene Name:ADCY9
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Adenylate cyclase type 9(ADCY9
Alternative Name:ADCY9,Adenylate cyclase type 9(ADCY9
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Adenylate cyclase type 9, ADCY9 ELISA KIT allows for the in vitro quantitative determination of Adenylate cyclase type 9(ADCY9 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Adenylate cyclase type 9, ADCY9 ELISA KIT has been pre-coated with an Adenylate cyclase type 9(ADCY9 antibody specific to Adenylate cyclase type 9(ADCY9 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Adenylate cyclase type 9(ADCY9 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Adenylate cyclase type 9(ADCY9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Adenylate cyclase type 9(ADCY9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!