Human Adenylosuccinate synthetase isozyme 2, ADSS ELISA KIT
Product Name:Human Adenylosuccinate synthetase isozyme 2, ADSS ELISA KIT
Packing:96T
Catalog No.:ELI-35857h
Gene Name:ADSS
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Adenylosuccinate synthetase isozyme 2(ADSS
Alternative Name:ADSS,Adenylosuccinate synthetase isozyme 2(ADSS
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Adenylosuccinate synthetase isozyme 2, ADSS ELISA KIT allows for the in vitro quantitative determination of Adenylosuccinate synthetase isozyme 2(ADSS concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Adenylosuccinate synthetase isozyme 2, ADSS ELISA KIT has been pre-coated with an Adenylosuccinate synthetase isozyme 2(ADSS antibody specific to Adenylosuccinate synthetase isozyme 2(ADSS .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Adenylosuccinate synthetase isozyme 2(ADSS and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Adenylosuccinate synthetase isozyme 2(ADSS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Adenylosuccinate synthetase isozyme 2(ADSS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!