Human AKT1 ELISA Kit

Product Name:Human AKT1 ELISA Kit
Packing:96T

Catalog No.:ELA-E0382h

Gene Name:AKT1

Detect Range:0.78-50 ng/mL

Sensitivity:0.195 ng/mL

Target Protein Name:Human RAC-alpha serine/threonine-protein kinase

Alternative Name:RAC-alpha serine/threonine-protein kinase, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, PKB, RAC
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human AKT1 ELISA Kit allows for the in vitro quantitative determination of Human RAC-alpha serine/threonine-protein kinase concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human AKT1 ELISA Kit has been pre-coated with an AKT1 antibody specific to Human RAC-alpha serine/threonine-protein kinase .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human RAC-alpha serine/threonine-protein kinase and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human RAC-alpha serine/threonine-protein kinase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm