Human Alkaline ceramidase 3, ACER3 ELISA KIT

Product Name:Human Alkaline ceramidase 3, ACER3 ELISA KIT
Packing:96T

Catalog No.:ELI-34998h

Gene Name:ACER3

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Alkaline ceramidase 3(ACER3

Alternative Name:ACER3,Alkaline ceramidase 3(ACER3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Alkaline ceramidase 3, ACER3 ELISA KIT allows for the in vitro quantitative determination of Alkaline ceramidase 3(ACER3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Alkaline ceramidase 3, ACER3 ELISA KIT has been pre-coated with an Alkaline ceramidase 3(ACER3 antibody specific to Alkaline ceramidase 3(ACER3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Alkaline ceramidase 3(ACER3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Alkaline ceramidase 3(ACER3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Alkaline ceramidase 3(ACER3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!