Human Alpha-enolase, ENO1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

ENO1,ENO, enolase ,ENO1L1; MPB1; NNE; PPH; 2-phospho-D-glycerate hydro-lyase; MYC promoter-binding protein 1; alpha enolase like 1; enolase-alpha; non-neural enolase; phosphopyruvate hydratase; plasminogen-binding protein; tau-crystallin; enolase 1 alpha,Alpha-enolase, MPB-1,MBP-1,Enolase1, Phosphopyruvate hydratase, C-myc promoter-binding protein

Search name

Human ENO1 ELISA KIT ,Human ENO ELISA KIT ,Human enolase ELISA KIT ,Human ENO1L1 ELISA KIT ,Human MPB1 ELISA KIT ,Human NNE ELISA KIT ,Human PPH ELISA KIT ,Human 2-phospho-D-glycerate hydro-lyase ELISA KIT ,Human MYC promoter-binding protein 1 ELISA KIT ,Human alpha enolase like 1 ELISA KIT ,Human enolase-alpha ELISA KIT ,Human non-neural enolase ELISA KIT ,Human phosphopyruvate hydratase ELISA KIT ,Human plasminogen-binding protein ELISA KIT ,Human tau-crystallin ELISA KIT ,Human enolase 1 alpha ELISA KIT ,Human Alpha-enolase ELISA KIT ,Human  MPB-1 ELISA KIT ,Human MBP-1 ELISA KIT ,Human Enolase1 ELISA KIT ,Human Phosphopyruvate hydratase ELISA KIT ,Human  C-myc promoter-binding protein ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human Alpha-enolase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-enolase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Alpha-enolase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Alpha-enolase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Alpha-enolase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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