Human Alpha- globin transcription factor CP2, TFCP2 ELISA KIT
Product Name:Human Alpha- globin transcription factor CP2, TFCP2 ELISA KIT
Packing:96T
Catalog No.:ELI-17230h
Gene Name:TFCP2
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Alpha-globin transcription factor CP2(TFCP2
Alternative Name:TFCP2,Alpha-globin transcription factor CP2(TFCP2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Alpha- globin transcription factor CP2, TFCP2 ELISA KIT allows for the in vitro quantitative determination of Alpha-globin transcription factor CP2(TFCP2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Alpha- globin transcription factor CP2, TFCP2 ELISA KIT has been pre-coated with an Alpha-globin transcription factor CP2(TFCP2 antibody specific to Alpha-globin transcription factor CP2(TFCP2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Alpha-globin transcription factor CP2(TFCP2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Alpha-globin transcription factor CP2(TFCP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Alpha-globin transcription factor CP2(TFCP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!