Human ALX homeobox protein 1, ALX1 ELISA KIT
Product Name:Human ALX homeobox protein 1, ALX1 ELISA KIT
Packing:96T
Catalog No.:ELI-24176h
Gene Name:ALX1
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:ALX homeobox protein 1(ALX1
Alternative Name:ALX1,ALX homeobox protein 1(ALX1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human ALX homeobox protein 1, ALX1 ELISA KIT allows for the in vitro quantitative determination of ALX homeobox protein 1(ALX1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human ALX homeobox protein 1, ALX1 ELISA KIT has been pre-coated with an ALX homeobox protein 1(ALX1 antibody specific to ALX homeobox protein 1(ALX1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ALX homeobox protein 1(ALX1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ALX homeobox protein 1(ALX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of ALX homeobox protein 1(ALX1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Technical Principle & Methodology
The quality assurance and experimental performance of this biological reagent are rigorously optimized and standardized according to its specific technical pathway:
Double Antibody Sandwich Method
(双抗体夹心法). This ensures maximum reproducibility across clinical research and scientific workflows.