Human AMD1 ELISA Kit
Product Name:Human AMD1 ELISA Kit
Packing:96T
Catalog No.:ELA-E0499h
Gene Name:AMD1
Detect Range:0.625-40 ng/mL
Sensitivity:0.38ng/mL
Target Protein Name:Human S-adenosylmethionine decarboxylase proenzyme
Alternative Name:S-adenosylmethionine decarboxylase proenzyme, AdoMetDC, SAMDC, AMD1, AMD
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human AMD1 ELISA Kit allows for the in vitro quantitative determination of Human S-adenosylmethionine decarboxylase proenzyme concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human AMD1 ELISA Kit has been pre-coated with an AMD1 antibody specific to Human S-adenosylmethionine decarboxylase proenzyme .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human S-adenosylmethionine decarboxylase proenzyme and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human S-adenosylmethionine decarboxylase proenzyme, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm