Human angiotension II ELISA Kit

Product Name:Human angiotension II ELISA Kit
Packing:96T

Catalog No.:ELA-E0005h

Gene Name:ANG-II

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:angiotension II

Alternative Name:ANG-II,angiotension II
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human angiotension II ELISA Kit allows for the in vitro quantitative determination of angiotension II concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human angiotension II ELISA Kit has been pre-coated with an angiotension II antibody specific to angiotension II .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for angiotension II and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain angiotension II, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of angiotension II in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!