Human ANKRD30A ELISA Kit

Product Name:Human ANKRD30A ELISA Kit
Packing:96T

Catalog No.:ELA-E1588h

Gene Name:ANKRD30A

Detect Range:0.156-10 ng/mL

Sensitivity:0.089ng/mL

Target Protein Name:Human Ankyrin repeat domain-containing protein 30A

Alternative Name:Ankyrin repeat domain-containing protein 30A, Serologically defined breast cancer antigen NY-BR-1, ANKRD30A
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human ANKRD30A ELISA Kit allows for the in vitro quantitative determination of Human Ankyrin repeat domain-containing protein 30A concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human ANKRD30A ELISA Kit has been pre-coated with an ANKRD30A antibody specific to Human Ankyrin repeat domain-containing protein 30A .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Ankyrin repeat domain-containing protein 30A and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Ankyrin repeat domain-containing protein 30A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm