Human APOA5 ELISA Kit

Product Name:Human APOA5 ELISA Kit
Packing:96T

Catalog No.:ELA-E1828h

Gene Name:APOA5

Detect Range:0.78-50 ng/mL

Sensitivity:0.67ng/mL

Target Protein Name:Human Apolipoprotein A-V

Alternative Name:Apolipoprotein A-V, Apo-AV, ApoA-V, Apolipoprotein A5, Regeneration-associated protein 3, APOA5, RAP3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human APOA5 ELISA Kit allows for the in vitro quantitative determination of Human Apolipoprotein A-V concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human APOA5 ELISA Kit has been pre-coated with an APOA5 antibody specific to Human Apolipoprotein A-V .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Apolipoprotein A-V and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Apolipoprotein A-V, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Apolipoprotein A-V in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!