Human Apolipoprotein E, ApoE ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Apolipoprotein E(Apo-E); ApoE,apo-E; AD2; LDLCQ5; LPG; apolipoprotein E3; apolipoprotein E

Search name

Human Apolipoprotein E ELISA Kit, Human ApoE ELISA Kit, Human Apo-E ELISA Kit, Human AD2 ELISA Kit, Human LDLCQ5

ELISA Kit, Human LPG ELISA Kit, Human apolipoprotein E3 ELISA Kit

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human apolipoprotein e,Apo-E concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apo-E. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Apo-E and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Apo-E, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Apo-E in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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