Human apoprotein B100 ELISA Kit
Product Name:Human apoprotein B100 ELISA Kit
Packing:96T
Catalog No.:ELA-E0603h
Gene Name:apo-B100
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:apoprotein B100
Alternative Name:apo-B100,apoprotein B100
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human apoprotein B100 ELISA Kit allows for the in vitro quantitative determination of apoprotein B100 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human apoprotein B100 ELISA Kit has been pre-coated with an apoprotein B100 antibody specific to apoprotein B100 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for apoprotein B100 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain apoprotein B100, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of apoprotein B100 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!