Human apoptosis protease activating factor- 1 ELISA Kit

Product Name:Human apoptosis protease activating factor- 1 ELISA Kit
Packing:96T

Catalog No.:ELA-E1054h

Gene Name:Apaf-1

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:apoptosis protease activating factor-1

Alternative Name:Apaf-1,apoptosis protease activating factor-1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human apoptosis protease activating factor- 1 ELISA Kit allows for the in vitro quantitative determination of apoptosis protease activating factor-1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human apoptosis protease activating factor- 1 ELISA Kit has been pre-coated with an apoptosis protease activating factor-1 antibody specific to apoptosis protease activating factor-1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for apoptosis protease activating factor-1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain apoptosis protease activating factor-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of apoptosis protease activating factor-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!