Human Arginine, Arg ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Arginine, Arg,R,2-Amino-5-guanidinopentanoic acid

Search name
Human Arg ELISA Kit, Human Arginine ELISA Kit, Human 2-Amino-5-guanidinopentanoic acid ELISA Kit, Human R ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human arginine,Arginine concentrations in serum, Plasma,
tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arginine. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Arginine and Avidin conjugated
to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain Arginine, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Arginine in the samples is then determined by comparing
the O.D. of the samples to the standard curve.

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