Human ATOH1 ELISA Kit

Product Name:Human ATOH1 ELISA Kit
Packing:96T

Catalog No.:ELA-E0255h

Gene Name:ATOH1

Detect Range:0.78-50 ng/mL

Sensitivity:0.33ng/mL

Target Protein Name:Human Protein atonal homolog 1

Alternative Name:Protein atonal homolog 1, Class A basic helix-loop-helix protein 14, bHLHa14, Helix-loop-helix protein hATH-1, hATH1, ATOH1, ATH1, BHLHA14
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human ATOH1 ELISA Kit allows for the in vitro quantitative determination of Human Protein atonal homolog 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human ATOH1 ELISA Kit has been pre-coated with an ATOH1 antibody specific to Human Protein atonal homolog 1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Protein atonal homolog 1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Protein atonal homolog 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm