Human ATP7A ELISA Kit

Product Name:Human ATP7A ELISA Kit
Packing:96T

Catalog No.:ELA-E15035h

Gene Name:ATP7A

Detect Range:0.156-10 ng/mL

Sensitivity:0.095ng/ml

Target Protein Name:Human Copper-transporting ATPase 1

Alternative Name:Copper-transporting ATPase 1, Copper pump 1, Menkes disease-associated protein, ATP7A, MC1, MNK
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human ATP7A ELISA Kit allows for the in vitro quantitative determination of Human Copper-transporting ATPase 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human ATP7A ELISA Kit has been pre-coated with an ATP7A antibody specific to Human Copper-transporting ATPase 1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Copper-transporting ATPase 1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Copper-transporting ATPase 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Copper-transporting ATPase 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!