Human AZGP1 ELISA Kit
Product Name:Human AZGP1 ELISA Kit
Packing:96T
Catalog No.:ELA-E2245h
Gene Name:AZGP1
Detect Range:1.56-100 ng/mL
Sensitivity:0.39ng/ml
Target Protein Name:Human Zinc-alpha-2-glycoprotein
Alternative Name:Zinc-alpha-2-glycoprotein, Zn-alpha-2-GP, Zn-alpha-2-glycoprotein, AZGP1, ZAG, ZNGP1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human AZGP1 ELISA Kit allows for the in vitro quantitative determination of Human Zinc-alpha-2-glycoprotein concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human AZGP1 ELISA Kit has been pre-coated with an AZGP1 antibody specific to Human Zinc-alpha-2-glycoprotein .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Zinc-alpha-2-glycoprotein and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Zinc-alpha-2-glycoprotein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Zinc-alpha-2-glycoprotein in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!