Human BACE1 ELISA Kit

Product Name:Human BACE1 ELISA Kit
Packing:96T

Catalog No.:ELA-E0718h

Gene Name:BACE1

Detect Range:0.78 ng/mL -50.0 ng/mL

Sensitivity:0.31ng/mL

Target Protein Name:Human Beta-secretase 1

Alternative Name:Membrane-associated aspartic protease 2;Memapsin-2;Aspartyl protease 2(ASP2;Asp 2);Beta-site amyloid precursor protein cleaving enzyme 1(Beta-site APP cleaving enzyme 1)
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human BACE1 ELISA Kit allows for the in vitro quantitative determination of Human Beta-secretase 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human BACE1 ELISA Kit has been pre-coated with an BACE1 antibody specific to Human Beta-secretase 1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Beta-secretase 1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Beta-secretase 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm