Human BCL11B ELISA Kit

Product Name:Human BCL11B ELISA Kit
Packing:96T

Catalog No.:ELA-E14966h

Gene Name:BCL11B

Detect Range:78-5000 pg/mL

Sensitivity:23 pg/ml

Target Protein Name:Human B-cell lymphoma/leukemia 11B

Alternative Name:B-cell lymphoma/leukemia 11B, BCL-11B, B-cell CLL/lymphoma 11B, COUP-TF-interacting protein 2, Radiation-induced tumor suppressor gene 1 protein, hRit1, BCL11B, CTIP2, RIT1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human BCL11B ELISA Kit allows for the in vitro quantitative determination of Human B-cell lymphoma/leukemia 11B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human BCL11B ELISA Kit has been pre-coated with an BCL11B antibody specific to Human B-cell lymphoma/leukemia 11B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human B-cell lymphoma/leukemia 11B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human B-cell lymphoma/leukemia 11B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human B-cell lymphoma/leukemia 11B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!