Human Bestrophin- 3, BEST3 ELISA KIT

Product Name:Human Bestrophin- 3, BEST3 ELISA KIT
Packing:96T

Catalog No.:ELI-24486h

Gene Name:BEST3

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Bestrophin-3(BEST3

Alternative Name:BEST3,Bestrophin-3(BEST3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Bestrophin- 3, BEST3 ELISA KIT allows for the in vitro quantitative determination of Bestrophin-3(BEST3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Bestrophin- 3, BEST3 ELISA KIT has been pre-coated with an Bestrophin-3(BEST3 antibody specific to Bestrophin-3(BEST3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bestrophin-3(BEST3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bestrophin-3(BEST3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bestrophin-3(BEST3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!