Human Beta- defensin 119, DEFB119 ELISA KIT

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

DEFB119,DEFB-19; DEFB-20; DEFB120; DEFB20; ESC42-RELA; ESC42-RELB; beta-defensin 119; defensin beta 120; defensin beta 19; defensin beta 20; defensin beta 119,Beta-defensin 20,Beta-defensin 120,Beta-defensin 19

Search name

Human DEFB119 ELISA KIT ,Human DEFB-19 ELISA KIT ,Human DEFB-20 ELISA KIT ,Human DEFB120 ELISA KIT ,Human DEFB20 ELISA KIT ,Human ESC42-RELA ELISA KIT ,Human ESC42-RELB ELISA KIT ,Human beta-defensin 119 ELISA KIT ,Human defensin beta 120 ELISA KIT ,Human defensin beta 19 ELISA KIT ,Human defensin beta 20 ELISA KIT ,Human defensin beta 119 ELISA KIT ,Human Beta-defensin 20 ELISA KIT ,Human Beta-defensin 120 ELISA KIT ,Human Beta-defensin 19 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human Beta-defensin 119 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-defensin 119, During the reaction, Beta-defensin 119 in the sample or standard competes with a fixed amount of biotin-labeled Beta-defensin 119 for sites on a pre-coated Monoclonal antibody specific to Beta-defensin 119. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Beta-defensin 119 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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