Human B- lymphocyte antigen CD20, MS4A1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

MS4A1, CD20,Bp35; CVID5; LEU-16; CD20 antigen; CD20 receptor; B-lymphocyte antigen CD20,Membrane-spanning 4-domains subfamily A member 1;Leukocyte surface antigen Leu-16;B-lymphocyte surface antigen B1

Search name

Human MS4A1 ELISA KIT ,Human CD20 ELISA KIT ,Human Bp35 ELISA KIT ,Human CVID5 ELISA KIT ,Human LEU-16 ELISA KIT ,Human CD20 antigen ELISA KIT ,Human CD20 receptor ELISA KIT ,Human B-lymphocyte antigen CD20 ELISA KIT ,Human Membrane-spanning 4-domains subfamily A member 1 ELISA KIT; Human Leukocyte surface antigen Leu-16 ELISA KIT; Human B-lymphocyte surface antigen B1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human B- lymphocyte antigen CD20, MS4A1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human B-lymphocyte antigen CD20,MS4A1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Human B-lymphocyte antigen CD20,MS4A1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human B-lymphocyte antigen CD20,MS4A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human B-lymphocyte antigen CD20,MS4A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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