Human Bradykinin ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

Human Bradykinin ELISA KIT ,Human KNG1 ELISA KIT ,Human BDK ELISA KIT ,Human KNG ELISA KIT ,Human kininogen-1 ELISA KIT ,Human HMWK ELISA KIT ,Human alpha-2-thiol proteinase inhibitor ELISA KIT ,Human fitzgerald factor ELISA KIT ,Human high molecular weight kininogen ELISA KIT ,Human williams-Fitzgerald-Flaujeac factor ELISA KIT ,Human kininogen 1 ELISA KIT

Intended use

Human Bradykinin ELISA kit allows for the in vitro quantitative determination of Human Bradykinin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

Human Bradykinin ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Bradykinin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bradykinin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Bradykinin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Human Bradykinin ELISA kit  Range 7.813-500pg/ml

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