Human BRO1 domain- containing protein BROX, BROX ELISA KIT
Product Name:Human BRO1 domain- containing protein BROX, BROX ELISA KIT
Packing:96T
Catalog No.:ELI-11140h
Gene Name:BROX
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:BRO1 domain-containing protein BROX(BROX
Alternative Name:BROX,BRO1 domain-containing protein BROX(BROX
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human BRO1 domain- containing protein BROX, BROX ELISA KIT allows for the in vitro quantitative determination of BRO1 domain-containing protein BROX(BROX concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human BRO1 domain- containing protein BROX, BROX ELISA KIT has been pre-coated with an BRO1 domain-containing protein BROX(BROX antibody specific to BRO1 domain-containing protein BROX(BROX .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for BRO1 domain-containing protein BROX(BROX and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain BRO1 domain-containing protein BROX(BROX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of BRO1 domain-containing protein BROX(BROX in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!