Human C4BPA ELISA Kit
Product Name:Human C4BPA ELISA Kit
Packing:96T
Catalog No.:ELA-E0761h
Gene Name:C4BPA
Detect Range:1.56-100 ng/mL
Sensitivity:0.56ng/mL
Target Protein Name:Human C4b-binding protein alpha chain
Alternative Name:C4b-binding protein alpha chain, C4bp, Proline-rich protein, PRP, C4BPA, C4BP
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human C4BPA ELISA Kit allows for the in vitro quantitative determination of Human C4b-binding protein alpha chain concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human C4BPA ELISA Kit has been pre-coated with an C4BPA antibody specific to Human C4b-binding protein alpha chain .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human C4b-binding protein alpha chain and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human C4b-binding protein alpha chain, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm