Product Name:Human C4BPA ELISA KIT
Product Name:Human C4BPA ELISA KIT
Gene Name:Human C4BPA
Detect Range:0.312-20ng/ml
Sensitivity:0.1ng/ml
Target Protein Name:Human C4BPA
Alternative Name:Human C4BPA
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human C4BPA ELISA KIT allows for the in vitro quantitative determination of Human C4BPA concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human C4BPA ELISA KIT has been pre-coated with an Human C4BPA antibody specific to Human C4BPA .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human C4BPA and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human C4BPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Human C4BPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!