Human Calcium uniporter protein, mitochondrial, MCU ELISA KIT

Product Name:Human Calcium uniporter protein, mitochondrial, MCU ELISA KIT
Packing:96T                                                Manual

Catalog No.:ELI-20463h

Gene Name:MCU

Detect Range:0.31-20ng/mL

Sensitivity:0.15ng/mL

Target Protein Name:Calcium uniporter protein(mitochondrial(MCU

Alternative Name:MCU,Calcium uniporter protein(mitochondrial(MCU
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Calcium uniporter protein, mitochondrial, MCU ELISA KIT allows for the in vitro quantitative determination of Calcium uniporter protein(mitochondrial(MCU concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Calcium uniporter protein, mitochondrial, MCU ELISA KIT has been pre-coated with an Calcium uniporter protein(mitochondrial(MCU antibody specific to Calcium uniporter protein(mitochondrial(MCU .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Calcium uniporter protein(mitochondrial(MCU and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Calcium uniporter protein(mitochondrial(MCU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Calcium uniporter protein(mitochondrial(MCU in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!