Human Carbohydrate sulfotransferase 12, CHST12 ELISA KIT

Product Name:Human Carbohydrate sulfotransferase 12, CHST12 ELISA KIT
Packing:96T

Catalog No.:ELI-25644h

Gene Name:CHST12

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Carbohydrate sulfotransferase 12(CHST12

Alternative Name:CHST12,Carbohydrate sulfotransferase 12(CHST12
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Carbohydrate sulfotransferase 12, CHST12 ELISA KIT allows for the in vitro quantitative determination of Carbohydrate sulfotransferase 12(CHST12 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Carbohydrate sulfotransferase 12, CHST12 ELISA KIT has been pre-coated with an Carbohydrate sulfotransferase 12(CHST12 antibody specific to Carbohydrate sulfotransferase 12(CHST12 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Carbohydrate sulfotransferase 12(CHST12 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Carbohydrate sulfotransferase 12(CHST12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Carbohydrate sulfotransferase 12(CHST12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!