Human Cardiac myosin- light chains 1 ELISA Kit

Product Name:Human Cardiac myosin- light chains 1 ELISA Kit
Packing:96T

Catalog No.:ELA-E1391h

Gene Name:CMLC-1

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Cardiac myosin-light chains 1

Alternative Name:CMLC-1,Cardiac myosin-light chains 1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Cardiac myosin- light chains 1 ELISA Kit allows for the in vitro quantitative determination of Cardiac myosin-light chains 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Cardiac myosin- light chains 1 ELISA Kit has been pre-coated with an Cardiac myosin-light chains 1 antibody specific to Cardiac myosin-light chains 1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cardiac myosin-light chains 1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cardiac myosin-light chains 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Cardiac myosin-light chains 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!