Human cardiac troponin I ELISA Kit
Product Name:Human cardiac troponin I ELISA Kit
Packing:96T
Catalog No.:ELA-E0478h
Gene Name:cTn-I
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:cardiac troponin I
Alternative Name:cTn-I,cardiac troponin I
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human cardiac troponin I ELISA Kit allows for the in vitro quantitative determination of cardiac troponin I concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human cardiac troponin I ELISA Kit has been pre-coated with an cardiac troponin I antibody specific to cardiac troponin I .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for cardiac troponin I and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain cardiac troponin I, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of cardiac troponin I in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!