Human caspase 4- apoptosis- related cysteine peptidase ELISA Kit

Product Name:Human caspase 4- apoptosis- related cysteine peptidase ELISA Kit
Packing:96T

Catalog No.:ELA-E1735h

Gene Name:Casp4

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:caspase 4-apoptosis-related cysteine peptidase

Alternative Name:Casp4,caspase 4-apoptosis-related cysteine peptidase
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human caspase 4- apoptosis- related cysteine peptidase ELISA Kit allows for the in vitro quantitative determination of caspase 4-apoptosis-related cysteine peptidase concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human caspase 4- apoptosis- related cysteine peptidase ELISA Kit has been pre-coated with an caspase 4-apoptosis-related cysteine peptidase antibody specific to caspase 4-apoptosis-related cysteine peptidase .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for caspase 4-apoptosis-related cysteine peptidase and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain caspase 4-apoptosis-related cysteine peptidase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of caspase 4-apoptosis-related cysteine peptidase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!