Human Caspase- 8, CASP8 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CASP8,caspase-8,ALPS2B; CAP4; Casp-8; FLICE; MACH; MCH5; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; ICE-like apoptotic protease 5; MACH-alpha-1/2/3 protein; MACH-beta-1/2/3/4 protein; MORT1-associated ced-3 homolog; apoptotic cysteine protease; apoptotic protease Mch-5; caspase 8, apoptosis-related cysteine protease; caspase 8, apoptosis-related cysteine peptidase
Search name
Human CASP8 ELISA KIT ,Human caspase-8 ELISA KIT ,Human ALPS2B ELISA KIT ,Human CAP4 ELISA KIT ,Human Casp-8 ELISA KIT ,Human FLICE ELISA KIT ,Human MACH ELISA KIT ,Human MCH5 ELISA KIT ,Human FADD-homologous ICE/CED-3-like protease ELISA KIT ,Human FADD-like ICE ELISA KIT ,Human ICE-like apoptotic protease 5 ELISA KIT ,Human MACH-alpha-1/2/3 protein ELISA KIT ,Human MACH-beta-1/2/3/4 protein ELISA KIT ,Human MORT1-associated ced-3 homolog ELISA KIT ,Human apoptotic cysteine protease ELISA KIT ,Human apoptotic protease Mch-5 ELISA KIT ,Human caspase 8 ELISA KIT ,Human apoptosis-related cysteine protease ELISA KIT ,Human caspase 8 ELISA KIT ,Human apoptosis-related cysteine peptidase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human caspase-8,CASP-8 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to CASP-8. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CASP-8 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CASP-8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CASP-8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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