Human Caspase-2,CASP2 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY!

Alternative name

Human Casp2 ELISA KIT ,Human CASP-2 ELISA KIT ,Human Caspase-2 ELISA KIT ,Human ICH1 ELISA KIT ,Human NEDD-2 ELISA KIT ,Human NEDD2 ELISA KIT ,Human PPP1R57 ELISA KIT ,Human caspase 2 apoptosis-related cysteine peptidase ELISA KIT ,Human neural precursor cell expressed developmentally down-regulated protein 2 ELISA KIT ,Human protease ICH-1 ELISA KIT ,Human protein phosphatase 1 ELISA KIT ,Human  regulatory subunit 57 ELISA KIT ,Human caspase 2 ELISA KIT

Intended use

Human Casp2 ELISA KIT allows for the in vitro quantitative determination of human caspase-2,CASP2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to CASP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CASP2 and Avidin conjugated to Horseradish Peroxidase (HRP)is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CASP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CASP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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