Human Cathepsin F, CTSF ELISA KIT

Product Name:Human Cathepsin F, CTSF ELISA KIT
Packing:96T

Catalog No.:ELI-50263h

Gene Name:CTSF

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Cathepsin F(CTSF

Alternative Name:CTSF,Cathepsin F(CTSF
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Cathepsin F, CTSF ELISA KIT allows for the in vitro quantitative determination of Cathepsin F(CTSF concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Cathepsin F, CTSF ELISA KIT has been pre-coated with an Cathepsin F(CTSF antibody specific to Cathepsin F(CTSF .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cathepsin F(CTSF and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cathepsin F(CTSF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Cathepsin F(CTSF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!