Human Cathepsin O, CTSO ELISA KIT

Product Name:Human Cathepsin O, CTSO ELISA KIT
Packing:96T

Catalog No.:ELI-49167h

Gene Name:CTSO

Detect Range:0.312-20 ng/mL

Sensitivity:0.039ng/mL

Target Protein Name:Cathepsin O(CTSO

Alternative Name:CTSO,Cathepsin O(CTSO
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Cathepsin O, CTSO ELISA KIT allows for the in vitro quantitative determination of Cathepsin O(CTSO concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Cathepsin O, CTSO ELISA KIT has been pre-coated with an Cathepsin O(CTSO antibody specific to Cathepsin O(CTSO .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cathepsin O(CTSO and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cathepsin O(CTSO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Cathepsin O(CTSO in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!