Human CCL18 ELISA Kit
Product Name:Human CCL18 ELISA Kit
Packing:96T
Catalog No.:ELA-E0211h
Gene Name:CCL18
Detect Range:0.156-10 ng/mL
Sensitivity:0.77ng/mL
Target Protein Name:Human C-C motif chemokine 18
Alternative Name:C-C motif chemokine 18, Alternative macrophage activation-associated CC chemokine 1, AMAC-1, CC chemokine PARC, Dendritic cell chemokine 1, DC-CK1, Macrophage inflammatory
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human CCL18 ELISA Kit allows for the in vitro quantitative determination of Human C-C motif chemokine 18 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human CCL18 ELISA Kit has been pre-coated with an CCL18 antibody specific to Human C-C motif chemokine 18 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human C-C motif chemokine 18 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human C-C motif chemokine 18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm